Protein isolation Lab Report Example | Topics and Well Written Essays - 500 words

Protein isolation - Lab Report Example The aim of the experiment is to isolate, purify and identify Rubsico in spinach leaves using Ammonium sulphate, spectrophotometry, and electrophoresis and ion exchange techniques. Spinach leaves were described, homogenized and rubisco isolated using two grades of ammonium sulphate concentrations (37 % and 50%). Filtrates and supernatants were tested using appropriate techniques to ascertain successful isolation. (Robinson, Streusand, Chatfield and Portis, 1988) The isolate was then purified using DEAE Cellulose fast flow ion exchange chromatography and bound proteins eluted using different shades of salt concentration. Purified proteins were run on a SDS-gel electrophoresis to identify the isolate (Robinson, Streusand, Chatfield and Portis , 1988). Ammonium sulphate (salt) precipitates proteins by altering hydrogen bond interaction between protein and water molecules. Ammonium sulphate a high affinity for water molecules displaces protein molecules (lowers the solubility) thereby causing precipitation. Different protein molecules precipitate at different concentrations of ammonium sulphate and at the rate at which it is added. The effect of salt concentration on the isolation of rubisco is shown on figure 1. The absence of the rubisco band on sample p2 (protein isolated at 50 % salt concentration) shows a significant amount of the protein was isolated at 37% concentrations of ammonium sulphate. A protein molecular weight ladder shown in figure 2 was used to establish a calibration curve that used to identify the protein isolate. Figure 1 The effect of salt concentration on the isolation of proteins; the top arrows represent the samples ( from left to right: MW ladder, leaf extract filtrate, Supernatant of first precipitate (S37%), Pellet of first precipitate (S37%) at low salt, Pellet of first precipitate (S37%) at medium salt, Pellet of

Mario Escobar, Matias Ramos, and Stephanie Solis Assignment

Mario Escobar, Matias Ramos, and Stephanie Solis - Assignment Example The paper tells that Matias Ramos, who was not eligible, for in-state tuition and his parents. They were not able to rise for his fees struggled hard until he was able to complete his four-year course in college. Stephanie Solis too had financial difficulties when she enrolled to UCLA. She decided to become a housecleaner; she made cardboard boxes and tutoring which helped her save money that enabled her to get through UCLA. The Langston Hughes poem, â€Å"A Dream Deferred† reflects a dream, which achievement is by hard work. The poem relates to the stories of Matias Ramos, Mario Escobar, and Stephanie Solis since their dreams were achieved through a tough struggle and hardships. In the poem, the dream is a load that is so heavy to bear and can not let one go a milestone to achieving the desired goals in life. The dream in the poem is full of pain, sorrow, and hardships that are not worthy to bear and can only be achieved by one who can only live the hard way out. As in the ca se of Mario Escobar who after surviving the El Salvador's brutal civil war where he lost his parents, he never gave up but struggled the hardest way out to achieve his dreams. The same applies to Stephanie Solis and Matias Ramos whose dreams were like a heavy load that had no signs of exploding, but through struggle, they are at the top of their dreams.

Gender Analysis of Sports Presenters

Gender Analysis of Sports Presenters CHAPTER 5 DISCUSSION The mindset of public towards the gender roles in sports Based on the findings in Chapter 4, the mindset of public in Kuala Lumpur towards the gender roles in sports is more open-minded in general. This means that the public in Kuala Lumpur believed that sports are not only males’ roles but it could be belonged to females too. However, there is only Gunther et al.’s (2011) study to support that the mindset of the public towards gender roles in sports is generally open-minded. From the findings, it is shown that the mindset of public disagreed that females are less knowledgeable and less competitive in sports as compare to males. This is supported by the study of Gunther et al. (2011), whereby the results shown that the public do not agree that females are less knowledgeable than males in sports. However, the findings of this study is contrasted by the study by Short (2012), in which it was mentioned that female sports broadcasters were seen as less competent and less knowledgeable in sports which showed contrast with the results of this study. In an interview, Kremer (2011) also mentioned that public often criticized that women do not know about sports, which also contradicted with the findings of this study. Although the mindset of public in Kuala Lumpur is open-minded, they believed males are the dominants and males are stronger in sports. This is supported by the outcome in the study of Centre for Gender Equality in Iceland (2006), which has clearly stated that sports have practically predominated by males or to say the attention is traditionally focused on males. Besides, the public in Kuala Lumpur also agreed that females show less interest in sports as compared to males. It is supported by the statement in Shine’s (2013) article where women are perceived as not interested and do not have the skills in sports. This could be because gender discrimination in sports has not been eliminated, which caused females not reaching a certain status in the public’s eye. In sum, the knowledge and the competency level of females in sports are not criticized in terms of their sex; however, males are still perceived as stronger and the dominants in sports. The reasons of males dominate in sports profession As the public perceived males are the dominants in sports profession, some reasons have been find out in this research. Based on the findings in this research, the reason of males dominates in sports profession is highly because males are biologically stronger than females. This is supported by the study conducted by Paulas (2013) where males are biologically stronger because of the structure of their body which differs from women. The research by Centre for Gender Equality in Iceland (2006) stated that biological nature is part of the consideration for males and females in participating sports. As sports emphasize a lot on physical strengths, females might be physically incompetent to participate in some sports because they are weak in strength. Toro and Sargent (2013) also concluded that males have the muscularity and superiority in sports because they run faster and jump faster than females. Males are overall more superior in sports. The public in Kuala Lumpur think that traditional gender roles are another major reason which leads to domination of males in sports profession. McMahon (2011) had given a statement that the nature of sports is masculine which is hardly related to feminine. Thus, this could be the reason that the public in Kuala Lumpur perceived sports as males’ gender roles. According to the research by Centre for Gender Equality in Iceland (2006), males have traditionally practiced to predominant in sports while females are not. In contrast, the public in Kuala Lumpur showed very slightly disagreed-to-neutral that sports show masculinity is the reason of males dominate in sports profession. According to Wilde’s study in 2007, sports are traditionally associated with males, masculinity and mainly dominated by the males. In the eyes of the public in Kuala Lumpur, sports maybe show masculinity, but that is not really a reason of why males dominate in sports profession. Moreover, the public in Kuala Lumpur does not perceive males as more superior in sports. In other words, both females and males are equal in sports. In the study of Paulas (2013), an argument has been raised up by saying that men are better than women in playing sports, which relates to the inherent biological features. However, females can be as skillful as males do because one’s skillfulness is related to how much instruction has taught to one in playing sports. In Grahame’s (2012) article also mentioned that, the biological differences in males’ body have made them more superior in most of the sports which differs from the opinion of the public in Kuala Lumpur. Besides, the public in Kuala Lumpur disagreed that the reason of males dominating in sports profession is because females are discourage to play sports. It is supported by Swanson (2009) where more females are encouraged to play sports after the enactment of Title IX in 1972 as compared to the past. 2012 Olympic Games, the women athletes have almost equal number as men and the ratio for athletes now is 50:50 male-female ratios (Gee, 2013). As Olympic Games is a worldwide sports, this could mean that females nowadays are encouraged to play sports universally. Furthermore, sports are not mainly designed for males in the view of the public in Kuala Lumpur. However, in the study by Paulas (2013), he found out that sports were designed for men at the first place. Sports such as basketball, football and baseball were created during the era where women were expected to stay in the kitchen and take care of the family. Sports were designed particularly based on males’ strengths and advantages where females can hardly beat males. As for most, the public in Kuala Lumpur do not believe that females are not competent in sports is the reason which caused males dominate in sports profession. Baiocchi-Wagner and Behm-Morawitz (2010) cited in their study that the public might be wrongly accused that females are less competent than males because males are better in playing sports. Also, Short (2012) also mentioned that women were seen as weak and less competent as compare to men which is not proven to be the truth. As nowadays more and more females started to step into sports profession, in which has proven that they are competent to compete with males in sports. Based on the open-ended data provided, the public perceived males are the dominant in sports because it is men’s interest. They are more passionate, enthusiast and willing to contribute their time in playing sports. In contrast, females do not like sports because they do not like to be exposed to the sun and sports are dirty and sweaty for them. In previous studies, it is shown that males show higher interest in participating, watching, and excelling in sports (Deaner et al., 2012). It concludes that males are more motivated to involve sports related activities which lead to the dominance of males in sports. An interesting reason that is concluded in the findings of this research is social and media influence. The preference of the social and media plays an important role in influencing the dominance of males in sports profession. The media coverage is more likely to focus on men’s sports than women’s sports. According to the study by Centre for Gender Equality in Iceland (2006), women’s sports are often treated as less important than men’s sports in media coverage. As a result, the public is able to be exposed to men’s sports more than women’s sports and to see males in sports has become a social expectation. In the study by Deaner et al. (2012) it was stated that sports interest is influenced by the societal gender roles, peers pressure, parents and so on. Thus, males will be more interested in participating sports while females have the social pressure to eschew sports. In the opinion of the public in Kuala Lumpur, education and parents’ influence also could be a reason of why males dominate in sports profession. As in the report by Garner (2012) stated that girls were being put off in Physical Education (PE) lesson because they have negative experience in participating PE such as being labeled as tomboy or unfeminine. Wetton et al. (2013) mentioned that the schools that favored boys’ sports activities have discourage girls’ attendance in participating sports. Other than that, parents do hold an important role in encouraging their children to involve in sports (Quinton, 2013). In the study by Wetton et al. (2013), the family background of the children and stereotypical view of parents do influenced children’s decision to not take part in sports. As the traditional mindset of gender roles in sports, parents might be more supportive for their sons to participate in sports but not for their daughters. Thus, there are more ma les involving themselves in sports than females due to the education and parents’ influence, in which has caused the dominance of males in sports profession. The level of opportunity of females to become sports broadcasters As concluded from the total scores of the level of opportunity for females to become sports broadcasters, the public in Kuala Lumpur believed that females have slightly high opportunity to become sports broadcasters. This has supported by Shine (2013) by saying that some organizations have started to hire talented female sports broadcasters because females can bring aspiring changes in sports broadcasting field. The public in Kuala Lumpur think that the opportunity is now given to females and females should be given equal opportunity to become sports broadcasters as males do. However, in the studies of Gunther et al. (2011) and Short (2012) showed that there are still inequalities in sports broadcasting field and this industry is lagging in creating equal opportunities for both males and females in becoming sports broadcasters. Although both of these studies are carried out in United States and England, but the public in Kuala Lumpur might be influenced by these leading countries. Besides, from the findings, it is shown that the public in Kuala Lumpur agreed that the opportunity for females on becoming sports broadcasters should be based on qualifications and skills rather than physical appearance. However, in the study of Gunther et al. (2011) found out that many stations are hiring attractive women regardless of their qualifications and skills just simply to retain their broadcasting license. The public acceptance of female sports broadcasters Moreover, this study has found out that the level of public acceptance of female sports broadcasters in Kuala Lumpur is high. The public did not particularly favored female or male sports broadcasters because they believed professionalism and knowledge of a sports broadcaster are important than one’s gender. Even for those major sports or male-dominated sports such as basketball and football, they do not think that only male sports broadcasters are good in reporting it. However, it is not supported by Shine (2013), where stated in her research that even though females are knowledgeable in male-dominated sports, the public will perceive females as do not have the skills to cover male-dominated sports. As in the study by Baiocchi-Wagner and Behm-Morawitz (2010) stated that women need to prove themselves twice to the public that they are worth as sports broadcasters. This has reflected that the acceptance of female in sports broadcasting field is very low. Many of the researchers have found out that the acceptance of female sports broadcasters were remaining low which contradict with this study. According to Futterman (2011), it has taken a long time for female sports broadcasters to be only partially accepted by the public. As long as gender discrimination and harassment still occurred, the acceptance of female in sports broadcasting will still low (Ebel, 2012). As time goes by, the difficulties or barriers for females have reduced, but the perception of the public on female sports broadcasters is stubbornly unchanged (Starphoenix, 2008).

Character Situations in One Day in the Life of Ivan Denisovich :: One Day in the Life of Ivan Denisovich Essays

Character Situations in One Day in the Life of Ivan Denisovich In One Day in the Life of Ivan Denisovich, Aleksandr Solzhenitsyn creates many characters that are memorable to the reader because of both their personal situations and their responses to those situations. Through characters such as Ivan Shukhov Denisovich, Fetyukov, Aloyska the Baptist, and the two Estonians, Solzhenitsyn explores the varied reactions of the characters and the effect of these reactions on other characters' perceptions of them. Ivan Shukhov Denisovich is the protagonist of this novel. He is the character about whom the reader is told the most. The reader is told that Denisovich has had several serious health problems, has been imprisoned for nearly ten years, and is a skilled laborer. Through conversations and actions it is learned that Shukhov, though not the highest ranking member of the prisoner group, is respected and well liked. His fellow prisoners care enough about him to watch his food for him when he is not present promptly at mealtimes and to give him the occasional drag off a cigarette. Much of this respect has to do with his attitude. Shukhov is willing to do many things for his fellow prisoners and does more than his share of the work. "He faces his situation positively," and tries to make use of any opportunity to assist the group as a whole (Depoli 1). The two Estonians are similar to Shukhov in many ways. They try to look at their situation in a positive light. They are brotherly and share everything to help make life a little bit better for each other. They cling together "as though neither would have air enough to breathe without the other," and they are respected for this devotion to each other (Solzhenitsyn 44). At one point Shukhov states that he has met many Estonians, including these two, "and never run across a bad one" (44). Because the two men try to work together, they are more highly thought of among the work gang than is someone like Fetyukov. Fetyukov is the scavenger. Seeking only to better his own situation, he is shunned by the other members of the group. He is referred to as a scoundrel and stoops to using odds and ends tossed away by other prisoners because "he got no help from anywhere" (45). Fetyukov is looked down upon by almost everyone in the group because of his selfishness and unwillingness to work. Character Situations in One Day in the Life of Ivan Denisovich :: One Day in the Life of Ivan Denisovich Essays Character Situations in One Day in the Life of Ivan Denisovich In One Day in the Life of Ivan Denisovich, Aleksandr Solzhenitsyn creates many characters that are memorable to the reader because of both their personal situations and their responses to those situations. Through characters such as Ivan Shukhov Denisovich, Fetyukov, Aloyska the Baptist, and the two Estonians, Solzhenitsyn explores the varied reactions of the characters and the effect of these reactions on other characters' perceptions of them. Ivan Shukhov Denisovich is the protagonist of this novel. He is the character about whom the reader is told the most. The reader is told that Denisovich has had several serious health problems, has been imprisoned for nearly ten years, and is a skilled laborer. Through conversations and actions it is learned that Shukhov, though not the highest ranking member of the prisoner group, is respected and well liked. His fellow prisoners care enough about him to watch his food for him when he is not present promptly at mealtimes and to give him the occasional drag off a cigarette. Much of this respect has to do with his attitude. Shukhov is willing to do many things for his fellow prisoners and does more than his share of the work. "He faces his situation positively," and tries to make use of any opportunity to assist the group as a whole (Depoli 1). The two Estonians are similar to Shukhov in many ways. They try to look at their situation in a positive light. They are brotherly and share everything to help make life a little bit better for each other. They cling together "as though neither would have air enough to breathe without the other," and they are respected for this devotion to each other (Solzhenitsyn 44). At one point Shukhov states that he has met many Estonians, including these two, "and never run across a bad one" (44). Because the two men try to work together, they are more highly thought of among the work gang than is someone like Fetyukov. Fetyukov is the scavenger. Seeking only to better his own situation, he is shunned by the other members of the group. He is referred to as a scoundrel and stoops to using odds and ends tossed away by other prisoners because "he got no help from anywhere" (45). Fetyukov is looked down upon by almost everyone in the group because of his selfishness and unwillingness to work.

The Educational System Trying

Is the guarantee of safety of others a compelling reason in which to form and maintain policies that make all school aged athletes or anyone who is involved in school based extra curricular activities subject to a mandatory drug screening or does this become a violation of constitutional rights? Can it be said that those in a choir or band recital pose just as much threat as those in vigorous sports and if so what kind of dangers do these people emit?Just how far can schools go in their policies befire they become shut down indefinitely when it comes to making policies â€Å"For the greater good? † It all started in 1995 when an Oregon school won their case in which they chose to make all athletes be drug tested. In an outcry the students filed suit and in the end it was the school who prevailed. Since then more and more schools are adopting or have done so or at least trying to adopt that same policy, many have succeeded with little to no issue while others have acted as the Acton family did in the Oregon case.Schools claim that in having a drug testing policy for athletes that this will help allay future endangerments and promote a healthier stance not only with the athletes but with the other students as well. Furthermore, the Oreogn school won their case for only those who are involved in athletics, not those that are involved in other extra curricular activities such as band or choir. The case in Oregon made in to the Supreme Court contenind violations of the fourth and fourteenth constitutional amendments.The Fourth amendment protects us against unreasonable search and seizures and that we are protected within our homes as well as the schools in which we attend and any other facility. The word reasonable has come into play through the course of arguments when arguing that forcing drug testing with no reasonable grounds went against this. With each case being different, the definition of reasonable also expands.The captain of a football team may be using as well as distributing and in this would lie reasonable cause for drug testing but the argument, as of 2000-2002, from yet another group challenging a school’s policy, is why should drug testing be enforced upon students when there is no reason visible per that student or group of students? But the Fourteenth amendment has also been cited; no person shall be deprived of life, liberty or property without due process of law and in forcing students to submit to drug testing simply because there is a policy allegedly violates this as suddenly they have been deprived of due process.So once again, another group of students have stepped forward, some outraged at the fact of a drug policy being implemented, not only for those who are athletes or in extra curricular activities but for all students, regardless of any exhibits or lack thereof of drug use and regardless of any known or unknown associations with any person who is thought to be a user. Simply, the case of Earls v. B oard of Education of Tecumseh Public School District claimed a definitive violation against them stoutly claiming the fourth amendment.In favbor of the Earls case, the Drug Policy Alliance Network filed a brief regarding this case, also contesting mandatory drug screens for all students. It has been claimed that sports actually is haven for drug free children and thus a protection. According to experts from the â€Å"American Academy of Pediatrics, the National Education Association, the American Public Health Association, the National Council on Alcoholism and Drug Dependence and several other prominent national organizations that thay all disagree with suspicionless drug testing of high school students engaged in extracurricular activities. † (http://www. drugpolicy.org/law/drugtesting/students/ 3rd paragraph). So with all of this, 10th Circuit held that the drug testing policy at issue violated students' Fourth Amendment rights but in 2002 the Supreme Court reversed that d ecision and upheld that school’s policy and then the ACLU took notice of this case and along with other similar cases, is challenging these violations of the violated constitutional rights. While the ACLU and circuit courts strike down the policies of schools with regards to their drug testing policies, the Supreme Court and the National Drug Control agency are for it and each side is pushing forward to be heard.Now the landmark of all of this is the fact that the 10th Circuit Court ruled against the Board of Education, the Supreme Court then came along and ruled back in favor of the Board of Education and in the same month the Supreme Court ruled against the Board of Education. A Supreme Court ruling against each other in the same month, unheard of. Justice Ginsburg in a subtle fashion held the school responsible more for their lack of proper concern and reasons in which to hold every child in punishment form for something that only some have done.He claimed this disregard a s if the tutelary responsibility was being taken a lot to far. To this day, this last decision has not been reversed. Any school intervention or other law enforcement intervention upon a student’s rights while at school must closely abide by the Vernonia, Oregon ruling of reasonableness. With these different factions going back and forth over what is quickly becoming an age old argument both sides have valid points but in the end it needs to be realized that drug testing students just because they can has proven to be of little to no use, not to mention a definite violation of legalities.Expert opinions of kids being driven away from sports due to this policy holds high validity. Where a child might be currently using, sports may also be the place that gets the child to stop using. In the end, regardless of testing, children will use and always have access to drugs should this be their decision and no school policy or court opinion will deter that. It hasn’t in hundred s of years and it isn’t about to start now. WORKS CITED BOARD OF ED. OF INDEPENDENT SCHOOL DIST. NO. 92 OF POTTAWATOMIE CTY. V. EARLS (01-332) 536 U. S.822 (2002) 242 F. 3d 1264, reversed. June 27, 2002 . http://www. law. cornell. edu/supct/html/01-332. ZD1. html NPR. Nina Totenburg. The Supreme Court and ‘Brown v. Board of Ed. Feb 6, 2009 http://www. npr. org/templates/story/story. php? storyId=1537409 Drug Testing Students. Drug Policy Alliance Network. 2009. Http://www. drugpolicy. org/law/drugtesting/students/ Reasonline. com. The Supreme Court's ruling on school drug testing will hurt public schools more than the one on vouchers. July 1, 2002. http://www. reason. com/news/show/32704.

Geographic coordinate system, Essay

Sextant- allowed users to determine their latitude to within a sea mile or two The gnomon or sun-shadow disk- operated like a sundial, enabling the user to determine his latitude by the length of the sun’s shadow cast on a disk floating level in water. The Arabian kamal- a rectangular plate that one moved closer or farther from one’s face until the distance between the North star and the horizon exactly corresponded to the plate’s upper and lower edges. The distance the plate lay away from the face—measured by a string tied to the center of the plate and held at the other end to the tip of the nose—determined the latitude. cross staff Tools used to measure longitude (Lesson 02.01) (use the first resource in this lesson to help you) Be able to list and describe the 2 main tools used to measure longitude in early navigation the chronometer- the sextant to figure longitude using the lunar-distance method Current Navigation Tools (Lesson 02.01) What tool do we currently use to determine location (latitude and longitude)? The Global Positioning System, Longitude and Latitude (Lesson 02.01) Know the ways that longitude and latitude run (N to S or E to W) and what they measure Longitude E to W – know how far east or west they were of the Prime Meridian Latidude N to S – how far north or south they were from the equator What are longitude and latitude measured in? they measure in degrees Challenger Expedition (Lesson 02.02)—Best resource link to use = â€Å"Account of the Voyage of the Challenger† Besides conducting sampling of the waters on its journey, what were the main findings from the Challenger voyage? found many new species, and provided collections for scores of biologists. What tool was used to do measure the depths of the ocean? The Challenger was equipped with 144 miles of sounding rope What are the 2 pieces of equipment used to collect bottom samples on the Challenger? At left is a shallow-water dredge, whereas a deep-sea trawl is shown at right. What was a newly invented piece of equipment used on the Challenger ship to get water samples called? Buchanan Water Sampler In which zone of the rocky shore would you most likely find only grasses and patches of lichens? splash zone What type of animal uses the rocky shore for their ‘feeding’ grounds? white-tailed deer in the winter What is the name of a plant-like species found in rocky shores? seaweed What is the special adaptation of these organisms found in the rocky shore ecosystem?—to do this, match up the following (Match the organism with its adaptation) Cky shore and tidal pools—what kinds of adaptations do they have that enable them to survive the pounding waves while staying in place, tidal changes (periods of time covered with water or periods of time with no water), sunlight exposure, evaporation of water that may leave concentrated salts behind, blending in to avoid predators What are the physical changes that occur in the rocky shore (3 main ones—think tides, salt content, and wave action)? Rock pools flat area of rock, back part forms a cliff where the front part is flat and in the water, formed by waves, wind, and rain Platform sloping shoreline where group of large rocks found on calm shorelines Boulder fields?depression left from a boulder grinding into a rock that hold water Crustaceans (Lessons 02.05 and 02.04) What 3 special adaptations do crustaceans have that allow them to survive in the rocky shore ecosystem? What characteristics do all crustaceans share (need to be specific) What is the best characteristic to help you identify a crustacean? What are the 3 main example types of crustaceans? Referenses: Baker, K. B., and S. Wing. â€Å"A new magnetic coordinate system for conjugate studies at high latitudes.† Journal of Geophysical Research: Space Physics 94.A7 (1989): 9139-9143. Dabek, Frank, et al. â€Å"Vivaldi: A decentralized network coordinate system.† ACM SIGCOMM Computer Communication Review. Vol. 34. No. 4. ACM, 2004. Gustafsson, G., N. E. Papitashvili, and V. O. Papitashvili. â€Å"A revised corrected geomagnetic coordinate system for Epochs 1985 and 1990.† Journal of atmospheric and terrestrial physics 54.11-12 (1992): 1609-1631. Priyantha, Nissanka B., et al. â€Å"Anchor-free distributed localization in sensor networks.† Proceedings of the 1st international conference on Embedded networked sensor systems. ACM, 2003. Goodchild, Michael F. â€Å"Citizens as sensors: the world of volunteered geography.† GeoJournal 69.4 (2007): 211-221.